Regulatory

Part:BBa_K1484323

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_NiI5, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 663
    Illegal SpeI site found at 219
    Illegal SpeI site found at 483
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 219
    Illegal SpeI site found at 483
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 663
    Illegal SpeI site found at 219
    Illegal SpeI site found at 483
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 663
    Illegal SpeI site found at 219
    Illegal SpeI site found at 483
    Illegal NgoMIV site found at 1989
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
None